Imagine one technology adaptable to so many process steps. Swapping out a fluid handling unit and process specific single-use cartridges makes it possible.
Whether you need to process an apheresis product or are harvesting from a bioreactor, the ekko system processes continuously without interruption. Flexible control allows for a wide range of inputs & final concentrations, yields or processing times. Peace of mind knowing that cell viability is maintained due to the low energy acoustic mechanism. Cell lines we have processed include:
T cell Harvest System Capabilities and Exemplar Results:
|Viable Cell Density||≥0.75e6 T cells/mL|
|Total Viable Cells||≥0.75e9 T cells|
|Viable Cell Recovery||90% ± 5%|
|Throughput||3 L/hr with additional 30 min wash|
Comparison of T cell population phenotypes before and after ekko™ cell processing. Phenotypes determined using flow cytometry show no impact of processing:
The ekko™ system can be connected to your bioreactor of choice to enable a closed, integrated, and automated solution for your PSC aggregate expansion or differentiation process. The system is tunable to maximize single cell removal or aggregate retention at each step while being gentle by minimizing time out of your reactor. Multiple unit operations can be performed such as:
Aggregate Pluripotent Stem Cell Media Exchange System Capabilities and Exemplar Results:
|Aggregate Cell Number||≥1e8 aggregate cells|
|Aggregate Recovery||Tunable up to 99%|
|Single Cell Depletion||Tunable up to 93%|
Output power titration curve on PSC aggregates with a size range of 100-200uM demonstrating impact of power/flow ratio on cluster retention (green) and single cell depletion (blue).
Removal of TCR+ cells in post-expansion allogeneic CAR-T process
Analysis demonstrates less than 3% TCR+ cells in single-pass process showing high purity of TCR- cells.
Cell Concentration determined using Nucleocounter NC-200, TCR determined using Flow cytometry
|Cell Surface Marker||Cell Concentration
Note: Results were obtained in under 2hr
Imagine a perfusion process without the need of filters. Our acoustic technology can do just that.